Zupancic T, Pihlar B
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia.
J Chromatogr A. 1999 Apr 23;840(1):11-20. doi: 10.1016/s0021-9673(99)00180-6.
The parameters influencing dialytic separation of ciprofloxacin (CF) fluoroquinolone were investigated. Dialysis with a porous cellulose acetate membrane was on-line coupled with HPLC and the analysis of dialysate was made by isocratic ion-pairing liquid chromatography using a reversed-phase analytical column and fluorescence detection. Optimisation of the experimental conditions for selective dialytic enrichment are described and explanations of some phenomena affecting dialysis efficiency discussed. By the use of a neutral donor (pH approximately 7) and acidic acceptor solution (pH < 4) a substantial enrichment of quinolones was achieved. Accumulation of CF in the acidic acceptor phase is based on the protonation of the analyte in the acceptor compartment. Continuous-flow of donor solution and a stagnant acceptor solution gave high dialysis efficiency in 5-15 min. Effects of interfering substances present in real samples on the variation of dialysis efficiency can be minimised by successive dialysis runs of the original and spiked samples.
研究了影响环丙沙星（CF）氟喹诺酮类药物透析分离的参数。使用多孔醋酸纤维素膜进行透析，并与高效液相色谱在线联用，通过等度离子对液相色谱法，使用反相分析柱和荧光检测对透析液进行分析。描述了选择性透析富集实验条件的优化，并讨论了一些影响透析效率的现象的解释。通过使用中性供体（pH约为7）和酸性受体溶液（pH < 4），实现了喹诺酮类药物的大量富集。CF在酸性受体相中的积累是基于分析物在受体隔室中的质子化。供体溶液的连续流动和停滞的受体溶液在5-15分钟内产生了高透析效率。通过对原始样品和加标样品进行连续透析运行，可以将实际样品中存在的干扰物质对透析效率变化的影响降至最低。
