一种用于同时测定猪粪便中恩诺沙星和环丙沙星的反相高效液相色谱分析法。
A reverse-phase HPLC assay for the simultaneous determination of enrofloxacin and ciprofloxacin in pig faeces.
作者信息
Sunderland Julie, Lovering Andrew M, Tobin Caroline M, MacGowan Alasdair P, Roe John M, Delsol Anne A
机构信息
Microbiology Department, Bristol Centre for Antimicrobial Research and Evaluation, Southmead Hostital, North Bristol NHS Trust, Bristol, BS10 5NB, UK.
出版信息
Int J Antimicrob Agents. 2004 Apr;23(4):390-3. doi: 10.1016/j.ijantimicag.2003.07.014.
A reverse-phase HPLC assay is described for the simultaneous assay of enrofloxacin (ENR) and ciprofloxacin (CPX) in pig faeces. Extraction used dichloromethane, 2-propanol and 0.3M ortho-phosphoric acid (1:5:4 v/v/v). Separation was achieved using a Spherisorb S5 C8 column, heated to 50 degrees C and a mobile phase of 0.16% ortho-phosphoric acid (adjusted to pH 3.0 with tetrabutylammonium hydroxide solution) with 20 ml acetonitrile per litre solution. The method used fluorescence detection (Ex 310 nm; Em 445 nm), a flow rate of 1 ml/min and a 20 microl injection volume. Retention times were approximately 6 min for ciprofloxacin and 10 min for enrofloxacin. The linearity range for both compounds was 0-20 mg/kg, lowest limit of quantification 0.3 mg/kg and recoveries were >92%.
本文描述了一种反相高效液相色谱法，用于同时测定猪粪便中恩诺沙星（ENR）和环丙沙星（CPX）的含量。萃取使用二氯甲烷、异丙醇和0.3M正磷酸（1:5:4 v/v/v）。采用Spherisorb S5 C8柱进行分离，柱温加热至50℃，流动相为0.16%正磷酸（用氢氧化四丁铵溶液调节至pH 3.0），每升溶液含20 ml乙腈。该方法采用荧光检测（激发波长310 nm；发射波长445 nm），流速为1 ml/min，进样体积为20 μl。环丙沙星的保留时间约为6分钟，恩诺沙星的保留时间约为10分钟。两种化合物的线性范围均为0 - 20 mg/kg，最低定量限为0.3 mg/kg，回收率>92%。
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