Takahashi M, Ishimaru N, Yanagi K, Saegusa K, Haneji N, Shiota H, Hayashi Y
Department of Ophthalmology, School of Medicine, University of Tokushima, Japan.
Clin Exp Immunol. 1999 May;116(2):231-7. doi: 10.1046/j.1365-2249.1999.00854.x.
Injection of antigen into the anterior chamber of the eye induces suppression of antigen-specific DTH, called anterior chamber-associated immune deviation (ACAID). It has been shown that the spleen is required for the induction of ACAID and detecting the ACAID-inducing signal from the eye. To examine the in vivo role of spleen cells, fractions of spleen cells were adoptively transferred into splenectomized mice. The present study showed that DTH was not suppressed in splenectomized mice, but was inhibited in splenectomized mice transferred with a primed CD4+ T cell-containing fraction of spleen cells. This indicates that the splenic CD4+ T cells comprise the regulatory T cells for the DTH response. When we examined the cytokine profile of the infiltrating T cells in the eye of primed mice by reverse transcriptase-polymerase chain reaction (RT-PCR), we found that they expressed IL-4, IL-10 mRNA (Th2 type), but not IL-2 and interferon-gamma (IFN-gamma) mRNA (Th1 type). By contrast, T cells which can elicit normal DTH response expressed IL-2 and IFN-gamma mRNA. These results suggest that splenic CD4+ T cells comprising the regulatory phenotype are required for the induction of ACAID, and that a DTH response to the antigen may be prevented by Th2-dominant CD4+ T cells.
将抗原注射到眼前房可诱导抗原特异性迟发型超敏反应（DTH）受到抑制，即前房相关免疫偏离（ACAID）。研究表明，诱导ACAID以及检测来自眼部的ACAID诱导信号均需要脾脏。为了研究脾细胞在体内的作用，将脾细胞组分过继转移到脾切除的小鼠体内。本研究表明，脾切除的小鼠中DTH未受到抑制，但在过继转移了含有经致敏的CD4⁺T细胞的脾细胞组分的脾切除小鼠中DTH受到了抑制。这表明脾脏CD4⁺T细胞构成了DTH反应的调节性T细胞。当我们通过逆转录聚合酶链反应（RT-PCR）检测经致敏小鼠眼部浸润T细胞的细胞因子谱时，发现它们表达IL-4、IL-10 mRNA（Th2型），但不表达IL-2和干扰素-γ（IFN-γ）mRNA（Th1型）。相比之下，能够引发正常DTH反应的T细胞表达IL-2和IFN-γ mRNA。这些结果表明，诱导ACAID需要具有调节表型的脾脏CD4⁺T细胞，并且Th2占主导的CD4⁺T细胞可能会阻止对抗原的DTH反应。
