Griffith T S, Herndon J M, Lima J, Kahn M, Ferguson T A
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA.
Int Immunol. 1995 Oct;7(10):1617-25. doi: 10.1093/intimm/7.10.1617.
Injection of antigen into the anterior chamber (AC) of the eye results in the induction of immune deviation in which antibody production is activated and delayed-type hypersensitivity (DTH) is inhibited. This system is termed anterior chamber associated immune deviation (ACAID) and the model is used to examine certain aspects of the immunologic privilege of the eye. Recent studies have established that following antigen presentation in the eye, an 'ACAID-inducing' signal is produced that directly enters the blood. This signal then homes to the spleen where T cells that down-regulate DTH are activated. For many antigens this 'ACAID signal' is a soluble protein released within 2 days of AC injection. Although the presence of this molecule (or molecules) has been described using several antigens, the exact nature of the soluble mediator has escaped characterization. We have further explored the nature of this signal using HSV-1-induced immune deviation. Our results show the soluble 'signal' was released by T cells that encounter antigen in the ocular microenvironment. This mediator was antigen specific, contained TCR alpha-chain (but not the TCR beta-chain) determinants and had an apparent molecular weight of 46 kDa. These results show that the release of soluble TCR alpha-chain from sites of T cell interaction within the microenvironment of the eye can regulate systemic immune responses. These results have implications for the control of immune response that might be damaging to organs such as the eye.
将抗原注入眼前房（AC）会导致免疫偏离的诱导，其中抗体产生被激活，迟发型超敏反应（DTH）受到抑制。这个系统被称为前房相关免疫偏离（ACAID），该模型用于研究眼睛免疫赦免的某些方面。最近的研究证实，在眼睛中呈现抗原后，会产生一种“诱导ACAID”的信号，该信号直接进入血液。然后该信号归巢至脾脏，在那里下调DTH的T细胞被激活。对于许多抗原来说，这种“ACAID信号”是在眼前房注射后2天内释放的一种可溶性蛋白质。尽管使用几种抗原描述了这种分子（或多种分子）的存在，但可溶性介质的确切性质仍未得到明确。我们使用单纯疱疹病毒1型（HSV-1）诱导的免疫偏离进一步探究了这种信号的性质。我们的结果表明，可溶性“信号”是由在眼部微环境中遇到抗原的T细胞释放的。这种介质具有抗原特异性，包含TCRα链（但不包含TCRβ链）决定簇，表观分子量为46 kDa。这些结果表明，在眼睛微环境中T细胞相互作用部位释放可溶性TCRα链可调节全身免疫反应。这些结果对于控制可能对眼睛等器官造成损害的免疫反应具有重要意义。
