Itoh M T, Ishizuka B, Kuribayashi Y, Amemiya A, Sumi Y
Department of Chemistry, St. Marianna University School of Medicine, Kawasaki, Japan.
Mol Hum Reprod. 1999 May;5(5):402-8. doi: 10.1093/molehr/5.5.402.
The presence of melatonin (N-acetyl-5-methoxytryptamine) and its precursors, serotonin (5-hydroxytryptamine) and N-acetylserotonin, was demonstrated in extracts of human ovary using reverse-phase high-performance liquid chromatography coupled with fluorometric detection. In addition, activities of two melatonin-synthesizing enzymes, arylalkylamine N-acetyltransferase (NAT) and hydroxyindole-O-methyltransferase (HIOMT), were found in human ovary homogenates. The apparent Michaelis constants for the substrates of NAT and HIOMT in the human ovary were similar to those reported for the pineal glands of humans and other mammals. These findings strongly suggest that the human ovary, like the pineal gland, may synthesize melatonin from serotonin by the sequential action of NAT and HIOMT.
利用反相高效液相色谱结合荧光检测法，在人卵巢提取物中证实了褪黑素（N - 乙酰 - 5 - 甲氧基色胺）及其前体血清素（5 - 羟色胺）和N - 乙酰血清素的存在。此外，在人卵巢匀浆中发现了两种褪黑素合成酶，芳基烷基胺N - 乙酰转移酶（NAT）和羟基吲哚 - O - 甲基转移酶（HIOMT）的活性。人卵巢中NAT和HIOMT底物的表观米氏常数与人类和其他哺乳动物松果体中报道的相似。这些发现有力地表明，人卵巢与松果体一样，可能通过NAT和HIOMT的顺序作用从血清素合成褪黑素。
