比较基因组杂交技术确定了人类间变性甲状腺癌中16号染色体短臂的频繁缺失。

Comparative genomic hybridization defines frequent loss on 16p in human anaplastic thyroid carcinoma.

作者信息

Komoike Y, Tamaki Y, Sakita I, Tomita N, Ohue M, Sekimoto M, Miyazaki M, Kadota M, Masuda N, Ooka M, Ohnishi T, Nakano Y, Kozaki T, Kobayashi T, Matsuura N, Ikeda T, Horii A, Monden M

机构信息

Department of Surgery II, Osaka University Medical School, Suita, Osaka 565-0871, Japan.

出版信息

Int J Oncol. 1999 Jun;14(6):1157-62. doi: 10.3892/ijo.14.6.1157.

DOI:10.3892/ijo.14.6.1157

PMID:10339673

Abstract

Anaplastic thyroid cancer (ATC) is one of the malignant tumors with the poor prognosis that is thought to arise from well-differentiated thyroid cancer (DTC). To investigate the molecular mechanism of ATC, we studied genomic alterations of eight ATC cell lines and three DTC cell lines by means of the comparative genomic hybridization (CGH) method. Loss of 16p was observed in five of eight ATC cell lines (62. 5%), but none of the three DTC cell lines showed loss of this chromosome arm. It is notable that loss of 18q [7/8 of ATC (87.5%), 2/3 of DTC (67%)] and gain of 20q [5/8 of ATC (62.5%), 3/3 of DTC (100%)] were frequently seen in both histologic types. Our results suggest that there is a gene in 16p that is closely associated with transformation from well-differentiated thyroid cancer to anaplastic cancer.

摘要

间变性甲状腺癌（ATC）是预后较差的恶性肿瘤之一，被认为起源于分化型甲状腺癌（DTC）。为了研究ATC的分子机制，我们通过比较基因组杂交（CGH）方法研究了8个ATC细胞系和3个DTC细胞系的基因组改变。在8个ATC细胞系中的5个（62.5%）观察到16p缺失，但3个DTC细胞系中均未出现该染色体臂的缺失。值得注意的是，18q缺失[ATC的7/8（87.5%），DTC的2/3（67%）]和20q增益[ATC的5/8（62.5%），DTC的3/3（100%）]在两种组织学类型中均常见。我们的结果表明，16p上存在一个与分化型甲状腺癌向间变性癌转化密切相关的基因。