Kang S J, Park K H, Park K S
Department of Science Education, College of Education, Ewha Womans University, Seoul, Korea.
Mol Cells. 1999 Apr 30;9(2):158-65.
The restriction maps of the 20 kb Amy region of Drosophila melanogaster in Korean populations were surveyed from 27 isogenic second chromosome lines. A subset of these populations were also scored for isozyme type and adult enzyme activity of alpha-amylase. Among 34 kinds of restriction site variations that were observed in the 20 kb region surrounding the two transcriptional units of the duplicated Amy locus, nine were polymorphic (26.5%). The low frequencies at which each of the large insertions was found are consistent with earlier reports of variation in other loci. Restriction site variation in Korean populations provided an estimated heterozygosity per nucleotide pair of 0.007. Three pairwise comparisons showed relatively high levels of linkage disequilibria. The enzyme activity of the Chounan population showed no significant difference compared with that of the Pusan population, and the activity of heterozygotes was higher than that of Amy1. Lines bearing insertion/deletion exhibited relatively low enzyme activities. All lines examined showed the duplication of Amy transcriptional units that were highly conserved, and the manner of concerted evolution.
从27个同基因的第二染色体品系中，对韩国果蝇群体中20kb Amy区域的限制酶切图谱进行了检测。还对这些群体的一个子集进行了同工酶类型和α-淀粉酶成年酶活性的评分。在围绕重复的Amy基因座的两个转录单位的20kb区域中观察到的34种限制酶切位点变异中，9种是多态性的（26.5%）。发现的每个大插入片段的低频与其他基因座变异的早期报告一致。韩国群体中的限制酶切位点变异估计每核苷酸对的杂合度为0.007。三次两两比较显示出相对较高水平的连锁不平衡。忠南群体的酶活性与釜山群体相比无显著差异，杂合子的活性高于Amy1。携带插入/缺失的品系表现出相对较低的酶活性。所有检测的品系都显示出高度保守的Amy转录单位的重复以及协同进化的方式。
