Gemmill R M, Schwartz P E, Doane W W
Nucleic Acids Res. 1986 Jul 11;14(13):5337-52. doi: 10.1093/nar/14.13.5337.
Restriction maps were made by Southern blot analysis of the Amy (alpha-amylase) region in 7 strains of D. melanogaster using endonucleases SalI, XhoI and EcoRI. These were compared to the map of lambda Dm65 which contains the cloned Amy region. Strains used produce either two amylase variants, a single variant, or no amylase, yet all 7 strains carry two Amy genes as inverted repeats at the Amy locus. This and the orientation of the repeats resembles the situation in lambda Dm65. Most restriction sites mapped are conserved but two strains contain a large insertion which differs in size and position between strains. A complex anomaly, probably an inversion, exists at the Amy locus in a null strain. Maps for our Amy1,3 strain and the lambda Dm65 clone are identical, the DNA of each having been derived from a Canton-S wild stock. Restriction and genetic maps of the Amy region were aligned and alleles assigned to the proximal and distal genes, Amy-p and Amy-d.
利用限制性内切酶SalI、XhoI和EcoRI，通过Southern印迹分析对7株黑腹果蝇的Amy（α-淀粉酶）区域进行了限制性图谱绘制。将这些图谱与含有克隆Amy区域的λDm65图谱进行了比较。所使用的菌株要么产生两种淀粉酶变体，要么产生单一变体，要么不产生淀粉酶，但所有7株菌株在Amy位点均携带两个作为反向重复的Amy基因。这一点以及重复序列的方向与λDm65中的情况相似。大多数绘制的限制性位点是保守的，但有两个菌株含有一个大的插入片段，不同菌株之间其大小和位置有所不同。在一个无淀粉酶菌株的Amy位点存在一个复杂的异常情况，可能是一个倒位。我们的Amy1,3菌株和λDm65克隆的图谱是相同的，二者的DNA均来源于Canton-S野生品系。对Amy区域的限制性图谱和遗传图谱进行了比对，并将等位基因分配给近端和远端基因Amy-p和Amy-d。
