Janssen D B, Counotte-Potman A D, Van Venrooij W J
Mol Biol Rep. 1976 Sep;3(1):87-95. doi: 10.1007/BF00357213.
Proteins present in messenger ribonucleoprotein particles were labeled with [35S]-methionine in Ehrlich ascites tumor cells in which synthesis of new ribosomes was inhibited. Poly(A)-protein complexes were isolated from free and membrane-bound polyribosomes by sucrose gradient centrifugation and affinity chromatography on oligo(dT)-cellulose. Both classes of Poly(A)-protein particles contain a poly(A) chain of about 70 adenyl residues and a protein with a molecular weight of 76000 attached to it.
在新核糖体合成受到抑制的艾氏腹水瘤细胞中，用[35S] - 甲硫氨酸标记信使核糖核蛋白颗粒中的蛋白质。通过蔗糖梯度离心和寡聚（dT） - 纤维素亲和层析从游离和膜结合的多核糖体中分离出聚（A） - 蛋白质复合物。两类聚（A） - 蛋白质颗粒都含有一条约70个腺苷残基的聚（A）链以及与之相连的分子量为76000的蛋白质。
