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大鼠肝细胞中含有多聚腺苷酸的核及多核糖体核糖核蛋白颗粒。

Nuclear and polyribosomal ribonucleoprotein particles containing poly (A) in rat liver cells.

作者信息

Tomcsanyi T, Molnar J, Tigyi A

出版信息

Mol Biol Rep. 1976 Nov;3(2):99-104. doi: 10.1007/BF00423222.

Abstract

Poly(A) containing ribonucleoprotein particles were prepared from rat liver nuclei and polyribosomes. The particles have sedimentation coefficients of 14 S and 9 S, respectively. In CS2SO4 density gradients the particles banded at densities of 1.28-1.29 g cm-3. Both nuclear and polyribosomal poly(A)-RNP contain in addition to some minor polypeptides, two main polypeptides having molecular weights of 63 000 and 90 000 dalton, respectively indistinguishable from each other according to their electrophoretic mobilities.

摘要

从大鼠肝细胞核和多核糖体中制备了含聚腺苷酸的核糖核蛋白颗粒。这些颗粒的沉降系数分别为14S和9S。在硫酸铯密度梯度中,颗粒在密度为1.28 - 1.29 g/cm³处形成条带。核内和多核糖体的聚腺苷酸核糖核蛋白除了一些次要多肽外,还含有两种主要多肽,其分子量分别为63000和90000道尔顿,根据它们的电泳迁移率彼此无法区分。

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