Murakami K, Horikawa K, Otsuki K
Pathology and Bacteriology Division, Fukuoka Institute of Health and Environmental Sciences, Japan.
J Vet Med Sci. 1999 Apr;61(4):439-42. doi: 10.1292/jvms.61.439.
To determine the extent of genetic diversity among isolates of Salmonella enteritidis obtained from outbreaks in Fukuoka Prefecture, Japan, from 1989 to 1994, we analyzed a total of 55 isolates of S. enteritidis obtained from 13 distinct outbreaks with pulsed-field gel electrophoresis. These isolates showed three different patterns in pulsed-field profile with Bln I digestion. The groups A, B and C consisted of three outbreaks isolates (Dice coefficient, F = 1), of seven outbreaks (F = 0.56-0.94) and of three outbreaks (F = 0.65-0.78), respectively. This result suggests that a few limited clonal lines of S. enteritidis were successively causing outbreaks in this area from 1989 to 1994.
为确定1989年至1994年从日本福冈县疫情中分离出的肠炎沙门氏菌菌株间的遗传多样性程度，我们用脉冲场凝胶电泳分析了从13起不同疫情中获得的总共55株肠炎沙门氏菌。这些菌株经Bln I酶切后在脉冲场图谱中呈现出三种不同模式。A、B和C组分别由三起疫情的分离株（相似系数，F = 1）、七起疫情的分离株（F = 0.56 - 0.94）和三起疫情的分离株（F = 0.65 - 0.78）组成。这一结果表明，1989年至1994年期间，少数有限的肠炎沙门氏菌克隆系在该地区相继引发疫情。
