Murase T, Nakamura A, Matsushima A, Yamai S
Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Japan.
Microbiol Immunol. 1996;40(11):873-5. doi: 10.1111/j.1348-0421.1996.tb01153.x.
An epidemiological analysis of Salmonella enteritidis from a food poisoning was done using pulsed-field gel electrophoresis (PFGE) of BlnI- or XbaI-digested fragments of chromosomal DNA of isolates. S. enteritidis isolates obtained from 19 patients had identical PFGE patterns. Therefore, a strain giving the same pattern was considered to be the causative agent of this outbreak. In addition, four isolates that had different BlnI-digested PFGE patterns were obtained from three patients, suggesting that the observed variations in PFGE patterns might occur as the result of some point mutations of chromosomal DNA during growth or from the existence of several S. enteritidis strains from various sources. Subsequent PFGE analysis of continuously subcultured strains supported the former possibility. These observations indicate that PFGE analysis on multiple numbers of colonies from each patient are necessary for the epidemiologic investigation of S. enteritidis.
通过对分离株染色体DNA经BlnI或XbaI酶切片段进行脉冲场凝胶电泳（PFGE），对一起食物中毒事件中的肠炎沙门氏菌进行了流行病学分析。从19名患者身上分离出的肠炎沙门氏菌菌株具有相同的PFGE图谱。因此，具有相同图谱的菌株被认为是此次疫情的病原体。此外，从3名患者身上获得了4株具有不同BlnI酶切PFGE图谱的分离株，这表明观察到的PFGE图谱差异可能是由于生长过程中染色体DNA的某些点突变，或者是由于存在来自不同来源的多种肠炎沙门氏菌菌株所致。随后对连续传代培养菌株进行的PFGE分析支持了前一种可能性。这些观察结果表明，对每名患者的多个菌落进行PFGE分析对于肠炎沙门氏菌的流行病学调查是必要的。
