通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳鉴定泰国牛体内的指形丝状线虫。

Setaria digitata in cattle of Thailand identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis.

作者信息

Subhachalat P, Shirasaka S, Nakajima H, Adachi Y

机构信息

Animal Health Laboratory, School of Agriculture, Ibaraki University, Japan.

出版信息

J Vet Med Sci. 1999 Apr;61(4):443-5. doi: 10.1292/jvms.61.443.

DOI:10.1292/jvms.61.443

PMID:10342300

Abstract

Adult Thai Setaria worms collected from cattle which were bred, housed and slaughtered in Thailand were morphologically identified as Setaria digitata. Furthermore, in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) adult Thai S. digitata had the same protein profiles as adult Japanese S. digitata, but did not possess the protein with a molecular size of 69 kDa which was confirmed in adult S. marshalli. In addition, there were no differences in the protein profiles between male and female S. digitata. In point of the distribution pattern of the proteins ranging from 73 to 64 kDa revealed by 2D-PAGE, there were no differences between Thai and Japanese S. digitata, and between male and female worms of the species.

摘要

从在泰国饲养、圈养和屠宰的牛身上采集的成年泰国丝状线虫在形态上被鉴定为指状丝状线虫。此外，在十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）中，成年泰国指状丝状线虫的蛋白质谱与成年日本指状丝状线虫相同，但不具有在成年马氏丝状线虫中得到证实的分子大小为69 kDa的蛋白质。此外，指状丝状线虫的雌雄两性在蛋白质谱上没有差异。就二维聚丙烯酰胺凝胶电泳（2D-PAGE）显示的73至64 kDa蛋白质的分布模式而言，泰国指状丝状线虫和日本指状丝状线虫之间以及该物种的雌雄虫之间均无差异。