Classification of Setaria worms from calves and adult cattle into Setaria digitata and Setaria marshalli by polyacrylamide gel electrophoresis.

The protein profiles of solubilized whole worms of the species, Setaria digitata and Setaria marshalli were compared using sodium dodecyl sulfate polyacrylamide gel electrophoresis. The results showed that one band with a molecular size of 69 kDa was confirmed only in S. marshalli, while this band was not detected in S. digitata. There were no differences of the major bands between males and females of the respective worm species. As further investigation, two-dimensional gel electrophoresis was performed and at least one spot ranging from 64 to 73 kDa was confirmed in S. marshalli but not in S. digitata. Therefore, those worms could be classified into two species biochemically as well as by the morphological characteristics.