Gassée J P, du Bois R, Staroukine M, Lambert P P
Pflugers Arch. 1976 Nov 30;367(1):15-24. doi: 10.1007/BF00583651.
The effects of the intrarenal infusion of synthetic Asn1, val 5 angiotensin II (AII) (from 0.38 to 1 mug min-1) on the determinants of glomerular filtration have been studied. The intracapillary and transcapillary pressure gradients along the capillaries, together with 2 parameters characterizing the porosity of the membrane in terms of pore theory (r, radius of the pores and Ap/1, total pore area per unit of path length) were calculated from the analysis of the sieving curve of I125 PVP molecules (polyvinylpyrrolidone) according to a biomathematical model previously described. AII increased PGC, the intracapillary hydrostatic pressure, but more at the efferent end of the capillaries. Filtration pressure equilibrium was maintained. AII also decreased the water permeability coefficient, Kf. by decreasing Ap/1, r remaining constant. Our results were compared to those obtained from the direct measurements of PGC, using micropuncture techniqles in hydropenic and plasma loaded rats. The complete agreement between the two approaches confirms the validity of the method based on the analysis of the sieving data of neutral macromolecules to calculate the determinants of GFR.
研究了肾内输注合成的天冬酰胺1、缬氨酸5血管紧张素II（AII）（速率为0.38至1微克/分钟）对肾小球滤过决定因素的影响。根据先前描述的生物数学模型，通过分析125I标记的聚乙烯吡咯烷酮（PVP）分子的筛分曲线，计算沿毛细血管的毛细血管内和跨毛细血管压力梯度，以及根据孔隙理论表征膜孔隙率的两个参数（r，孔隙半径和Ap/1，单位路径长度的总孔隙面积）。AII增加了PGC，即毛细血管内静水压力，但在毛细血管的出端增加得更多。滤过压力平衡得以维持。AII还通过降低Ap/1（r保持不变）降低了水渗透系数Kf。我们的结果与使用微穿刺技术在缺水和血浆负荷大鼠中直接测量PGC所获得的结果进行了比较。两种方法完全一致，证实了基于中性大分子筛分数据分析来计算肾小球滤过率决定因素的方法的有效性。
