Lukácová N, Jalc P, Marsala J
Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovak Republic.
Mol Chem Neuropathol. 1998 Aug-Dec;35(1-3):61-76. doi: 10.1007/BF02815116.
Changes in the concentration of membrane-bound phospholipids following single (25-min) spinal cord ischemia and 3 h of reperfusion were determined. These were compared with the changes following brief repeated (8-, 8-, and 9-min) ischemia followed each time by reperfusion for 1 h, or the same periods of ischemia followed by 8 h, 8 h, and 24 h of reperfusion, respectively. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), and sphingomyelin (SM) were assayed in regions of the spinal cord of the rabbit, including gray matter, white matter, dorsal horns, intermediate zone, and ventral horns. The brief repeated ischemia with 1-h reperfusions produced more extensive degradation of phospholipids in almost all regions compared with the equivalent time of ischemia (25 min) in a single period. After a lengthy reperfusion after repeated ischemia, the phospholipids were resynthesized with the exception of the phosphatidylinositol in the gray matter. The resynthesis was most pronounced in the dorsal horns and in the white matter.
测定了单次（25分钟）脊髓缺血及3小时再灌注后膜结合磷脂浓度的变化。将这些变化与短暂重复（8分钟、8分钟和9分钟）缺血每次继以1小时再灌注后的变化进行比较，或与相同缺血时间分别继以8小时、8小时和24小时再灌注后的变化进行比较。在兔脊髓的区域，包括灰质、白质、背角、中间带和腹角，对磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰丝氨酸（PS）、磷脂酰肌醇（PI）和鞘磷脂（SM）进行了测定。与单次25分钟缺血等效时间相比，短暂重复缺血并继以1小时再灌注在几乎所有区域都导致了更广泛的磷脂降解。重复缺血后长时间再灌注后，除灰质中的磷脂酰肌醇外，磷脂会重新合成。这种重新合成在背角和白质中最为明显。
