在抗氧化剂存在的情况下测定总胆汁酸反应性物质和烯醛。

The determination of TBA-reactive substances and alkenals in the presence of antioxidants.

作者信息

Balthazary S T, Sallmann H P, Fuhrmann H

机构信息

Department of Veterinary Physiology, Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, Morogoro, Tanzania.

出版信息

Acta Vet Hung. 1999;47(2):155-9. doi: 10.1556/004.47.1999.2.1.

DOI:10.1556/004.47.1999.2.1

PMID:10344076

Abstract

This study investigated the inhibitory efficiency of all-rac-alpha-tocopherol, 2,6-ditert-butyl-p-cresole (BHT), and 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carbonic acid (Trolox) on determination of thiobarbituric acid-reactive substances (TBARS) and short-chain alkenals in rat liver homogenates. The concentration of TBARS was measured fluorophotometrically. Aldehydes were determined after derivatization with methylhydrazine by gas chromatography (GLC). The concentrations of alkenals and TBARS in liver homogenates were diminished when antioxidants were present during the sample preparation. It is suggested that in the absence of antioxidants the samples are autoxidized further during the preparative procedures. For the aldehyde determination all-rac-alpha-tocopherol was the most effective antioxidant to reduce the bias due to autoxidation, whereas for TBARS it was Trolox.

摘要

本研究调查了消旋α-生育酚、2,6-二叔丁基对甲酚（BHT）和6-羟基-2,5,7,8-四甲基色满-2-羧酸（Trolox）对大鼠肝匀浆中硫代巴比妥酸反应性物质（TBARS）和短链烯醛测定的抑制效率。TBARS的浓度采用荧光光度法测定。醛类在用甲基肼衍生化后通过气相色谱法（GLC）测定。当在样品制备过程中存在抗氧化剂时，肝匀浆中烯醛和TBARS的浓度会降低。这表明在没有抗氧化剂的情况下，样品在制备过程中会进一步自动氧化。对于醛类测定，消旋α-生育酚是最有效的抗氧化剂，可减少因自动氧化导致的偏差，而对于TBARS，最有效的是Trolox。