Alabadí D, Carbonell J
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Spain.
Plant Mol Biol. 1999 Mar;39(5):933-43. doi: 10.1023/a:1006158819849.
Two cDNAs from young pea fruits coding for functional spermidine synthases (EC 2.5.1.16) were isolated. The corresponding genes were named psSPDSYN1 and psSPDSYN2. Both cDNAs complemented spe3delta gene when introduced into the Y480 strain of Saccharomyces cerevisiae, which is a null mutant for the spermidine synthase gene. psSPDSYN1 and psSPDSYN2 are regulated differentially. psSPDSYN1 is up-regulated early after fruit set whereas psSPDSYN2 is expressed later. Spermidine synthase activity was detected in pea ovaries, and correlates with the pattern of expression of psSPDSYN1. In the pea plant, psSPDSYN1 is highly expressed in actively growing tissues, whereas the highest level of psSPDSYN2 mRNA was detected in fully elongated stem.
从嫩豌豆果实中分离出了两个编码功能性亚精胺合酶（EC 2.5.1.16）的cDNA。相应的基因被命名为psSPDSYN1和psSPDSYN2。当将这两个cDNA导入酿酒酵母Y480菌株（该菌株是亚精胺合酶基因的缺失突变体）时，它们都能互补spe3delta基因。psSPDSYN1和psSPDSYN2受到不同的调控。psSPDSYN1在坐果后早期上调，而psSPDSYN2稍后表达。在豌豆子房检测到了亚精胺合酶活性，且其与psSPDSYN1的表达模式相关。在豌豆植株中，psSPDSYN1在活跃生长的组织中高表达，而psSPDSYN2 mRNA的最高水平则在完全伸长的茎中检测到。
