Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Jaime Roig 11, E-46010, Valencia, Spain.
Planta. 1989 Dec;178(4):482-7. doi: 10.1007/BF00963818.
Separation and quantitation of polyamines from unpollinated pea (Pisum sativum L.) ovaries and young fruits induced by application of gibberellic acid to unpollinated ovaries showed, in both cases, a decrease in putrescine and spermidine levels between anthesis and 4 d later. By contrast, spermine levels increased prior to the onset of senescence of the unpollinated ovaries (3 d post anthesis) and decreased during fruit development. Low levels of putrescine, spermidine and spermine were also observed in young fruits obtained by self-pollination and by treatment of unpollinated ovaries with 2,4-dichlorophenoxyacetic acid. In-vitro culture of ovary explants in a medium containing spermine showed that a reduction of the growth of gibberellic acid-treated unpollinated ovaries was associated with a rise in the level of spermine in the fruits. The results obtained indicate that changes in spermine levels are involved in the control of ovary senescence and of fruit set and development.
用赤霉素处理未授粉的豌豆（Pisum sativum L.）子房和幼果，可诱导未授粉的子房和幼果衰老，从中分离和定量多胺。结果表明，在授粉后 4d 内，无论是未授粉的子房还是幼果，腐胺和亚精胺的含量都有所下降。相比之下，在未授粉的子房衰老（授粉后 3d）之前，精胺水平增加，并在果实发育过程中下降。通过自花授粉和用 2,4-二氯苯氧乙酸处理未授粉的子房获得的幼果中，也观察到腐胺、亚精胺和精胺的含量较低。将子房外植体在含有精胺的培养基中进行体外培养的结果表明，用赤霉素处理的未授粉子房的生长减少与果实中精胺水平的升高有关。这些结果表明，精胺水平的变化可能参与了子房衰老、果实结实和发育的调控。
