Stoyanov A V, Righetti P G
Institute of Gene Biology, Russian Academy of Sciences, Moscow.
Electrophoresis. 1999 Apr-May;20(4-5):718-22. doi: 10.1002/(SICI)1522-2683(19990101)20:4/5<718::AID-ELPS718>3.0.CO;2-A.
The problem of stationary electrolysis of a solution of nonamphotheric compounds (acids or bases) is considered. The analysis is performed by taking into account the mobility dependence on pH. The properties of such a system are also compared with the ones pertaining to the solution of an amphoteric substance. It is anticipated that steady-state electrolysis of free acids and bases, in a convection-free system, might be useful for creating narrow pH gradients in rather acidic and alkaline milieus, which might be adopted for focusing without resorting to conventional carrier ampholytes or immobilized pH gradients.
考虑了非两性化合物（酸或碱）溶液的稳态电解问题。通过考虑迁移率对pH的依赖性进行分析。还将这种体系的性质与两性物质溶液的性质进行了比较。预计在无对流系统中，游离酸和碱的稳态电解可能有助于在相当酸性和碱性的环境中创建狭窄的pH梯度，这可用于聚焦，而无需使用传统的载体两性电解质或固定化pH梯度。
