不同类型隐形眼镜佩戴者与正常对照组泪液乳酸脱氢酶活性的比较。

Comparison of tear lactate dehydrogenase activities of different types of contact lens wearers and normal control group.

作者信息

Iskeleli G, Karakoc Y, Akdeniz-Kayhan B, Kayhan U, Gurler B, Ozkan S

机构信息

Department of Ophthalmology, Cerrahpasa Medical Faculty, University of Istanbul, Turkey.

出版信息

CLAO J. 1999 Apr;25(2):101-4.

PMID:10344295

Abstract

PURPOSE

The purpose of this study was to compare the lactate dehydrogenase (LDH) activities in the tears of patients wearing different types of contact lenses with each other and to individuals who have never worn contact lenses (controls).

METHODS

Tear LDH activities were determined in 13 normal subjects who had never worn contact lenses, 12 daily wear and ten extended wear soft contact lens wearers, and 14 daily wear and eight extended wear rigid gas permeable (RGP) contact lens wearers(Dk125). Tear samples were collected from all subjects at the same time of day from the inferior meniscus by using a microcapillary tube. The measurements were determined using a Sigma diagnostic LDH reagent and a spectrophotometer.

RESULTS

There was no statistically significant difference in the LDH activities between soft and RGP contact lenses--used either as daily or extended wear--when compared with each other. However, individuals wearing extended wear soft contact lenses had significantly higher tear LDH activities than the control group.

CONCLUSION

Tear LDH activity may be a useful method in evaluating the physiologic response of extended soft contact lens wear.

摘要

目的

本研究旨在比较佩戴不同类型隐形眼镜的患者与从未佩戴过隐形眼镜者（对照组）泪液中乳酸脱氢酶（LDH）的活性。

方法

对13名从未佩戴过隐形眼镜的正常受试者、12名日戴型和10名长戴型软性隐形眼镜佩戴者以及14名日戴型和8名长戴型硬性透气性（RGP）隐形眼镜（Dk125）佩戴者的泪液LDH活性进行了测定。使用微量毛细管在一天中的同一时间从所有受试者的下泪弯月处采集泪液样本。测量使用Sigma诊断LDH试剂和分光光度计进行。