Bouhafs R K, Jarstrand C
Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Huddinge Hospital, Karolinska Institute, Sweden.
Pediatr Pulmonol. 1999 May;27(5):322-7. doi: 10.1002/(sici)1099-0496(199905)27:5<322::aid-ppul5>3.0.co;2-e.
Surfactant therapy is given routinely to premature newborns with respiratory failure. However, alterations in surfactants have been shown to be a significant factor in some forms of respiratory failure in newborns in animal models of lung injury. To investigate whether antioxidant supplementation might help to protect exogenous surfactant from damage by oxygen free radicals, we examined the influence of vitamin E in combination with surfactant on superoxide production as estimated by the nitroblue tetrazolium reduction test, and measured surfactant peroxidation with a new colorimetric method with or without addition of superoxide dismutase (SOD) or vitamin E. Our results showed that surfactant interacts with free radicals; surfactant reduced superoxide production by neutrophils and was peroxidized when incubated with resting and with stimulated cells. Vitamin E supplementation decreased superoxide radical production and in a dose-dependent manner decreased surfactant peroxidation. The decrease in lipid peroxidation by SOD was not significant. These findings suggest that phagocytes induce lipid peroxidation of lung surfactant, a reaction that might be prevented by antioxidants.
表面活性剂疗法通常用于患有呼吸衰竭的早产新生儿。然而，在肺损伤动物模型中，已表明表面活性剂的改变是新生儿某些形式呼吸衰竭的一个重要因素。为了研究补充抗氧化剂是否有助于保护外源性表面活性剂免受氧自由基的损伤，我们通过硝基蓝四氮唑还原试验评估了维生素E与表面活性剂联合使用对超氧化物产生的影响，并使用一种新的比色法测量了添加或不添加超氧化物歧化酶（SOD）或维生素E时表面活性剂的过氧化作用。我们的结果表明，表面活性剂与自由基相互作用；表面活性剂可减少中性粒细胞产生的超氧化物，并且在与静息细胞和刺激细胞孵育时会发生过氧化。补充维生素E可减少超氧自由基的产生，并以剂量依赖的方式减少表面活性剂的过氧化。SOD对脂质过氧化的降低作用不显著。这些发现表明，吞噬细胞可诱导肺表面活性剂的脂质过氧化，而抗氧化剂可能会阻止这一反应。
