Verbert A, Cacan R, Montreuil J
Eur J Biochem. 1976 Nov 1;70(1):49-53. doi: 10.1111/j.1432-1033.1976.tb10954.x.
Experiments are described to demonstrate the existence of ectogalactosyltransferase activity on the lymphocyte surface. The procedures described enable us to exclude the possibility of misleading results due to precursor hydrolysis and intracellular utilization of the free galactose. This depicted transferase is able to catalyse the transfer of a galactosyl residue from UDP-galactose to a nonphagocytosable exogenous acceptor and to endogenous membrane acceptors. The cells galactosylated in this way acquired new agglutinating properties with soybean agglutinin, which proves the external position of the galactosyl residues incorporated on the cell surface.
本文描述了一些实验，以证明淋巴细胞表面存在外源性半乳糖基转移酶活性。所描述的实验步骤使我们能够排除由于前体水解和游离半乳糖的细胞内利用而导致误导性结果的可能性。所描述的这种转移酶能够催化半乳糖基残基从UDP-半乳糖转移到不可吞噬的外源性受体和内源性膜受体上。以这种方式进行半乳糖基化的细胞获得了与大豆凝集素结合的新凝集特性，这证明了结合在细胞表面的半乳糖基残基位于细胞外部。
