大豆凝集素对神经氨酸酶处理的淋巴细胞的转化作用
Transformation of neuraminidase-treated lymphocytes by soybean agglutinin.
作者信息
Novogrodsky A, Katchalski E
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2515-8. doi: 10.1073/pnas.70.9.2515.
Abstract
Transformation of mouse-spleen lymphocytes in the presence of soybean agglutinin is markedly enhanced after their treatment with neuraminidase (EC 3.2.1.18). Incubation of the cells with the enzyme also facilitates their agglutination by the lectin. The soybean agglutinin-induced agglutination and transformation is inhibited specifically by N-acetyl-D-galactosamine. New specific binding sites for soybean agglutinin were shown to appear after neuraminidase treatment. It is postulated that the transformation of neuraminidase-treated mouse-spleen lymphocytes induced by soybean agglutinin is caused by the binding of the lectin to galactosyl residues exposed by the action of neuraminidase on the cell membrane.
摘要
用神经氨酸酶（EC 3.2.1.18）处理小鼠脾脏淋巴细胞后，在大豆凝集素存在下其转化作用明显增强。细胞与该酶一起孵育也有助于它们被凝集素凝集。大豆凝集素诱导的凝集和转化被N-乙酰-D-半乳糖胺特异性抑制。经神经氨酸酶处理后，显示出现了大豆凝集素的新的特异性结合位点。据推测，大豆凝集素诱导的经神经氨酸酶处理的小鼠脾脏淋巴细胞的转化是由于凝集素与神经氨酸酶作用于细胞膜后暴露的半乳糖基残基结合所致。
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