Cook A R
J Gen Microbiol. 1976 Dec;97(2):235-40. doi: 10.1099/00221287-97-2-235.
A chemically-defined medium was developed which supported growth of Streptococcus faecium and permitted synthesis of urease. This streptococcus cannot utilize ammonia and needs a complex medium, but its requirements are probably provided in the rumen. The specific activity of urease was inversely related to growth and in no medium was there high growth and high urease activity. Anaerobic culture and the presence of urea in the medium were essential for urease activity, but not for growth.
开发了一种化学成分明确的培养基，该培养基支持粪肠球菌生长并允许脲酶合成。这种链球菌不能利用氨，需要复杂培养基，但其需求可能在瘤胃中得到满足。脲酶的比活性与生长呈负相关，在任何培养基中都不会同时出现高生长和高脲酶活性。厌氧培养和培养基中存在尿素对脲酶活性至关重要，但对生长并非如此。
