Kanoe M, Koyanagi Y, Kondo C, Mamba K, Makita T, Kai K
Laboratory of Animal Health, Tokyo University of Agriculture, Japan.
Microbios. 1998;96(383):33-8.
The location of haemagglutinin (HA) of Fusobacterium necrophorum subsp. necrophorum VPI 2891 strain was investigated by immunofluorescence, confocal laser scan microscopy and immunoelectron microscopy. The immunofluorescence study demonstrated the fluorescence specific for the HA on the bacterial cells and confocal laser scan microscopy indicated similar fluorescence around the cross section of the bacterial cell. The immunoelectron microscopic study also revealed that the protein A-gold conjugates were located around the bacterial surfaces. These findings suggest that HA is one of the components of the cell surfaces of F. necrophorum subsp, necrophorum.
通过免疫荧光、共聚焦激光扫描显微镜和免疫电子显微镜研究了坏死梭杆菌坏死亚种VPI 2891菌株血凝素（HA）的定位。免疫荧光研究显示细菌细胞上有HA特异性荧光，共聚焦激光扫描显微镜表明在细菌细胞横截面周围有类似荧光。免疫电子显微镜研究还显示蛋白A-金结合物位于细菌表面周围。这些发现表明HA是坏死梭杆菌坏死亚种细胞表面的组成成分之一。
