Schade S Z, Yotis W W, Gopalsami C, Simonson L G
Department of Applied Laboratory Science, Naval Dental Research Institute, Great Lakes, Illinois 60088-5259, USA.
Microbios. 1998;96(383):51-63.
The outer membrane (OM) was isolated by detergent extraction from Treponema denticola ATCC 35405, ATCC 33521 and ATCC 35404, representing serovars a, b and c, respectively, as well as from two fresh isolates of T. denticola. Strict precautions were undertaken against the introduction of contaminant lipopolysaccharide when the OM was isolated. The OM was active in mitogenic stimulation of C3H/HeOuJ mouse spleen cultures, but to a somewhat lesser extent than purified lipopolysaccharide (LPS) from Escherichia coli 055:B5. Polymyxin B only partially inhibited the response. Unheated OM abrogated mitogenic activity of E. coli LPS, but heated preparations enhanced the mitogenic activity of E. coli LPS, suggesting the presence of a heat-labile cytolytic factor associated with T. denticola OM in addition to a putative lipopolysaccharide and/or heat-stable lipoprotein.
从分别代表血清型a、b和c的具核梭杆菌ATCC 35405、ATCC 33521和ATCC 35404以及两株具核梭杆菌新鲜分离株中，通过去污剂提取法分离外膜（OM）。分离OM时采取了严格的预防措施以防止引入污染性脂多糖。OM对C3H/HeOuJ小鼠脾细胞培养物具有促有丝分裂刺激活性，但程度略低于来自大肠杆菌055:B5的纯化脂多糖（LPS）。多粘菌素B仅部分抑制该反应。未加热的OM消除了大肠杆菌LPS的促有丝分裂活性，但加热后的制剂增强了大肠杆菌LPS的促有丝分裂活性，这表明除了假定的脂多糖和/或热稳定脂蛋白外，具核梭杆菌OM还存在一种热不稳定的细胞溶解因子。
