Kurimoto T, Suzuki M, Watanabe T
Department of Microbiology, Nippon Dental University School of Dentistry at Tokyo.
Shigaku. 1990 Aug;78(2):208-32.
Lipopolysaccharides (LPSs) were isolated from Treponema denticola (T. denticola) and Treponema vincentii (T. vincentii) by the phenol/water (PW) and the phenol/chloroform/petroleum-ether (PCP) procedures. 1) T. denticola PW-LPS (LPS isolated by the PW procedure), PCP-sup-LPS (LPS isolated by the PCP procedure in the supernates of ultracentrifugation), PCP-ppt-LPS (precipitated LPS isolated by the PCP procedure, obtained after ultracentrifugation), and T. vincentii PCP-ppt-LPS were composed of carbohydrate, hexosamine, protein, fatty acid, and phosphorus. T. vincentii PW-LPS was contained major amount of carbohydrates and small amount of fatty acids. 2-keto-3-deoxyoctonic acid (KDD) was not detected in these LPSs. 2) The major fatty acids of T. denticola PW-LPS and PCP-sup-LPS were palmitic, stearic, oleic, and linoleic acids. The major fatty acids of T. vincentii PCP-ppt-LPS were palmitic, stearic, myristic, oleic, and linoleic acids. Hydroxy fatty acids were not detected. 3) Glucose, galactose, and mannose were comprised in T. denticola PW-LPS. Glucose, galactose, and arabinose were comprised in T. denticola PCP-sup-LPS. Glucose and galactose were comprised in T. vincentii PCP-ppt-LPS. 4) The Limulus amoebocyte lysate (LAL) clotting activity of T. denticola PW-LPS was 1/10, as compared with that of Escherichia coli (E. coli) UKT-B LPS standard. The LAL clotting activities of T. denticola PCP-sup-LPS, T. vincentii PW-LPS, and T. vincentii PCP-ppt-LPS were 1/100, as compared with that of E. coli LPS standard. 5) Five hundred micrograms/kg of T. denticola PCP-sup-LPS was pyrogenic in rabbits. Two thousand micrograms/kg of T. vincentii PCP-ppt-LPS was pyrogenic in rabbits. 6) T. denticola PCP-sup-LPS and T. vincentii PCP-ppt-LPS were capable of increasing or decreasing the release of lysosomal enzymes from human polymorphonuclear leukocytes.
通过苯酚/水（PW）法和苯酚/氯仿/石油醚（PCP）法从具核梭杆菌（T. denticola）和文森特氏密螺旋体（T. vincentii）中分离出脂多糖（LPSs）。1）具核梭杆菌PW-LPS（通过PW法分离的LPS）、PCP-上清-LPS（通过PCP法从超速离心上清液中分离的LPS）、PCP-沉淀-LPS（通过PCP法分离的沉淀LPS，超速离心后获得）以及文森特氏密螺旋体PCP-沉淀-LPS均由碳水化合物、己糖胺、蛋白质、脂肪酸和磷组成。文森特氏密螺旋体PW-LPS含有大量碳水化合物和少量脂肪酸。在这些LPS中未检测到2-酮-3-脱氧辛酸（KDD）。2）具核梭杆菌PW-LPS和PCP-上清-LPS的主要脂肪酸为棕榈酸、硬脂酸、油酸和亚油酸。文森特氏密螺旋体PCP-沉淀-LPS的主要脂肪酸为棕榈酸、硬脂酸、肉豆蔻酸、油酸和亚油酸。未检测到羟基脂肪酸。3）具核梭杆菌PW-LPS中含有葡萄糖、半乳糖和甘露糖。具核梭杆菌PCP-上清-LPS中含有葡萄糖、半乳糖和阿拉伯糖。文森特氏密螺旋体PCP-沉淀-LPS中含有葡萄糖和半乳糖。4）与大肠杆菌（E. coli）UKT-B LPS标准品相比，具核梭杆菌PW-LPS的鲎试剂（LAL）凝血活性为1/10。与大肠杆菌LPS标准品相比，具核梭杆菌PCP-上清-LPS、文森特氏密螺旋体PW-LPS和文森特氏密螺旋体PCP-沉淀-LPS的LAL凝血活性为1/100。5）500微克/千克的具核梭杆菌PCP-上清-LPS对家兔有热原性。2000微克/千克的文森特氏密螺旋体PCP-沉淀-LPS对家兔有热原性。6）具核梭杆菌PCP-上清-LPS和文森特氏密螺旋体PCP-沉淀-LPS能够增加或减少人多形核白细胞溶酶体酶的释放。
