Leemans I, Brown D, Hooshmand-Rad P, Kirvar E, Uggla A
Department of Parasitology, Swedish University of Agricultural Sciences and National Veterinary Institute, Uppsala.
Vet Parasitol. 1999 Apr 12;82(3):179-92. doi: 10.1016/s0304-4017(99)00013-8.
In a series of experiments, sporozoite stabilates of a Theileria lestoquardi (Lahr) and a T. annulata (Ankara) stock prepared from Hyalomma anatolicum anatolicum ticks, were used to examine the infectivity of both parasite species for sheep and cattle and to study the development of cross-immunity between these parasite species. In the first experiment sheep and cattle were inoculated with T. lestoquardi sporozoites. Surviving animals and naive sheep and cattle were, in the second experiment, inoculated with T. annulata. In the third experiment, naive sheep and sheep previously infected with T. annulata, were inoculated with T. lestoquardi. The following responses to inoculations were monitored: clinical and haematological signs of infection, appearance of parasitic stages of the parasites in lymph node biopsies and in peripheral blood and serological response to T. lestoquardi and T. annulata schizont antigens. While T. lestoquardi readily infected sheep and caused severe disease, it did not infect cattle. On the other hand, T. annulata infected both cattle and sheep. However, whereas cattle became severely affected, infected sheep showed mild clinical symptoms only and piroplasms did not develop. Despite their different behaviour in the host species examined, cross-immunity studies suggested that the parasite species are very closely related. Experiments in sheep indicated that T. lestoquardi infection protected against subsequent T. annulata infection. On the other hand, recovery from T. annulata infection did not prevent infection by sporozoites of T. lestoquardi, resulting in the establishment of schizonts and their subsequent development into piroplasms, although it protected against the major clinical effects of T. lestoquardi infection.
在一系列实验中，使用从安纳托利亚璃眼蜱制备的莱氏泰勒虫（Lahr）和环形泰勒虫（安卡拉株）的子孢子冻干品，来检测这两种寄生虫对绵羊和牛的感染性，并研究这两种寄生虫之间交叉免疫的发展情况。在第一个实验中，给绵羊和牛接种莱氏泰勒虫的子孢子。在第二个实验中，给存活下来的动物以及未接触过病原体的绵羊和牛接种环形泰勒虫。在第三个实验中，给未接触过病原体的绵羊以及先前感染过环形泰勒虫的绵羊接种莱氏泰勒虫。监测了以下接种反应：感染的临床和血液学体征、淋巴结活检和外周血中寄生虫寄生阶段的出现情况，以及对莱氏泰勒虫和环形泰勒虫裂殖体抗原的血清学反应。虽然莱氏泰勒虫很容易感染绵羊并引发严重疾病，但它不会感染牛。另一方面，环形泰勒虫能感染牛和绵羊。然而，牛受到的影响很严重，而感染的绵羊仅表现出轻微的临床症状，且未出现梨形虫。尽管在所检测的宿主物种中它们的表现不同，但交叉免疫研究表明这两种寄生虫关系非常密切。在绵羊身上进行的实验表明，莱氏泰勒虫感染可预防随后的环形泰勒虫感染。另一方面，从环形泰勒虫感染中恢复并不能预防莱氏泰勒虫子孢子的感染，尽管它能预防莱氏泰勒虫感染带来的主要临床影响，但仍会导致裂殖体的形成及其随后发育为梨形虫。
