Donovan J L, Luthria D L, Stremple P, Waterhouse A L
Department of Viticulture and Enology, University of California, Davis 95616, USA.
J Chromatogr B Biomed Sci Appl. 1999 Apr 16;726(1-2):277-83. doi: 10.1016/s0378-4347(99)00019-5.
(+)-Catechin and (-)-epicatechin are found in many foods and may have important effects on human health. These compounds, like many other catechols, are thought to be converted to methylated metabolites after ingestion. This paper describes the synthesis of the 3'- and 4'-methyl ethers and their unambiguous identification. These products, along with catechin, epicatechin and an internal standard, (+)-taxifolin, were separated using RP-HPLC with ultraviolet, electrochemical and fluorescence detection. The trimethylsilylated derivatives of the seven compounds were also separated by GC with mass spectrometric detection. The limits of detection and selectivity of the analytical methods were compared with respect to their application in complex matrices such as human plasma.
（+）-儿茶素和（-）-表儿茶素存在于许多食物中，可能对人体健康有重要影响。这些化合物与许多其他儿茶酚一样，被认为在摄入后会转化为甲基化代谢物。本文描述了3'-和4'-甲基醚的合成及其明确鉴定。这些产物与儿茶素、表儿茶素和内标（+）-紫杉叶素一起，使用反相高效液相色谱法结合紫外、电化学和荧光检测进行分离。这七种化合物的三甲基硅烷基化衍生物也通过气相色谱结合质谱检测进行分离。就其在人血浆等复杂基质中的应用，比较了分析方法的检测限和选择性。
