采用反相高效液相色谱法结合二极管阵列检测器（DAD）和硅烷化后气相色谱 - 质谱联用技术分析芳香植物中天然存在的酚类化合物。
Analysis of Naturally Occurring Phenolic Compounds in Aromatic Plants by RP-HPLC Coupled to Diode Array Detector (DAD) and GC-MS after Silylation.
作者信息
Proestos Charalampos, Komaitis Michael
机构信息
Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771, Athens, Greece.
Laboratory of Food Chemistry and Analysis, Department of Food Science and Technology, Agricultural University of Athens, 11855, Athens, Greece.
出版信息
Foods. 2013 Mar 13;2(1):90-99. doi: 10.3390/foods2010090.
The following aromatic plants of Greek origin, (dictamus), (eucalyptus), L. (oregano), L. (balm mint) and (mountain tea), were examined for the content of phenolic substances. Reversed phase HPLC coupled to diode array detector (DAD) was used for the analysis of the plant extracts. The gas chromatography-mass spectrometry method (GC-MS) was also used for identification of phenolic compounds after silylation. The most abundant phenolic acids were: gallic acid (1.5-2.6 mg/100 g dry sample), ferulic acid (0.34-6.9 mg/100 g dry sample) and caffeic acid (1.0-13.8 mg/100 g dry sample). (+)-Catechin and (-)-epicatechin were the main flavonoids identified in oregano and mountain tea. Quercetin was detected only in eucalyptus and mountain tea.
对以下原产于希腊的芳香植物（白鲜）、（桉树）、牛至属（牛至）、唇萼薄荷属（香脂薄荷）和（山茶花）进行了酚类物质含量的检测。采用反相高效液相色谱法结合二极管阵列检测器（DAD）对植物提取物进行分析。气相色谱 - 质谱联用方法（GC - MS）也用于硅烷化后酚类化合物的鉴定。含量最丰富的酚酸为：没食子酸（1.5 - 2.6毫克/100克干样品）、阿魏酸（0.34 - 6.9毫克/100克干样品）和咖啡酸（1.0 - 13.8毫克/100克干样品）。（+） - 儿茶素和（ - ） - 表儿茶素是在牛至和山茶花中鉴定出的主要黄酮类化合物。槲皮素仅在桉树和山茶花中检测到。
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