Winkler S, Willheim M, Baier K, Aichelburg A, Kremsner P G, Graninger W
Department of Internal Medicine I, and Institute of General and Experimental Pathology, University of Vienna, Austria.
Am J Trop Med Hyg. 1999 Apr;60(4):680-6. doi: 10.4269/ajtmh.1999.60.680.
The frequency of cytokine-producing peripheral blood mononuclear cells was assessed in 28 subjects with microfilaremic loiasis and in 14 amicrofilaremic individuals. In addition, a subgroup of seven microfilaremic individuals coinfected with Plasmodium malariae was evaluated. By using flow cytometry for the intracellular detection of cytokines, a more pronounced T helper (Th)2 cell-type response with the expansion of interleukin (IL)-4, IL-10, and IL-13 expressing CD4+ cells in the microfilaremic compared with the amicrofilaremic group was noted. Expression of IL-5 was equivalent in both groups as was the frequency of Th2-type cytokines expressing CD8+ cells and of Th1-type cytokines (interferon [IFN]-gamma, IL-2, IFN-gamma/IL-2) producing CD4+ and CD8+ cells. Th0-type cytokine-expressing cells, represented by IL-4/IFN-gamma, IL-10/IFN-gamma, and IL-13/IFN-gamma, were equally distributed within groups. Coinfection of P. malariae did not significantly alter the cytokine expression compared with microfilaremic individuals without P. malariae infections. By identifying a large panel of cytokine-producing T cell subpopulations, a Th2-driven immune response in microfilaremic Loa loa patients was noted.
在28名有微丝蚴血症的罗阿丝虫病患者和14名无微丝蚴血症的个体中评估了产生细胞因子的外周血单核细胞的频率。此外，还对7名同时感染了间日疟原虫的有微丝蚴血症个体的亚组进行了评估。通过使用流式细胞术对细胞内细胞因子进行检测，发现与无微丝蚴血症组相比，有微丝蚴血症组中表达白细胞介素（IL）-4、IL-10和IL-13的CD4+细胞扩增，呈现出更明显的辅助性T（Th）2细胞型反应。两组中IL-5的表达以及表达Th2型细胞因子的CD8+细胞和产生Th1型细胞因子（干扰素[IFN]-γ、IL-2、IFN-γ/IL-2）的CD4+和CD8+细胞的频率相当。以IL-4/IFN-γ、IL-10/IFN-γ和IL-13/IFN-γ为代表的表达Th0型细胞因子的细胞在各组中分布均匀。与未感染间日疟原虫的有微丝蚴血症个体相比，间日疟原虫的合并感染并未显著改变细胞因子的表达。通过鉴定大量产生细胞因子的T细胞亚群，发现有微丝蚴血症的罗阿丝虫病患者存在由Th2驱动的免疫反应。
