Hirose K, Kadowaki S, Tanabe M, Takeshima H, Iino M
Department of Pharmacology, Faculty of Medicine, University of Tokyo and CREST, Japan Science and Technology Corporation, Tokyo 113-8654, Japan.
Science. 1999 May 28;284(5419):1527-30. doi: 10.1126/science.284.5419.1527.
Inositol 1,4,5-trisphosphate (IP3) is a second messenger that elicits complex spatiotemporal patterns of calcium ion (Ca2+) mobilization and has essential roles in the regulation of many cellular functions. In Madin-Darby canine kidney epithelial cells, green fluorescent protein-tagged pleckstrin homology domain translocated from the plasma membrane to the cytoplasm in response to increased concentration of IP3. The detection of translocation enabled monitoring of IP3 concentration changes within single cells and revealed spatiotemporal dynamics in the concentration of IP3 synchronous with Ca2+ oscillations and intracellular and intercellular IP3 waves that accompanied Ca2+ waves. Such changes in IP3 concentration may be fundamental to Ca2+ signaling.
肌醇 1,4,5 -三磷酸（IP3）是一种第二信使，它引发钙离子（Ca2+）动员的复杂时空模式，并在许多细胞功能的调节中发挥重要作用。在麦迪逊 - 达比犬肾上皮细胞中，绿色荧光蛋白标记的普列克底物蛋白同源结构域会因IP3浓度升高而从质膜转移至细胞质。这种转移的检测能够监测单个细胞内IP3浓度的变化，并揭示与Ca2+振荡以及伴随Ca2+波的细胞内和细胞间IP3波同步的IP3浓度时空动态。IP3浓度的这种变化可能是Ca2+信号传导的基础。
