Ribas Antúnez M A, Arocha Rosete Y, Toraño Canto I, Rodríguez Valdés C
Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba.
Rev Cubana Med Trop. 1998;50(2):105-9.
60 matched sera clinically suspicious of measles that were received at the Diagnostic Laboratory of the "Pedro Kourí" Tropical Medicine Institute between January and May, 1996, coming from the seroepidemiological surveillance of the MPR vaccine were studied. The detection of measles and rubella hemagglutinant antibodies, as well as of IgM, was carried out with the Clark Laboratories INC. Measles IgM ELISA diagnostic kit. The positive cases were confirmed by the IgM-indirect immunofluorescence and neutralization. 3 positive cases to measles and rubella, which were negative to measles IgM, were obtained by hemagglutination inhibition. Antibodies against Epstein Barr, cytomegalovirus and herpes simplex virus were also determined by indirect immunofluorescence (IIF) due to the capacity of these viruses to induces polyclonal responses. 6 positive cases, which were negative by IIF, were detected by means of the above mentioned diagnostic kit.
对1996年1月至5月间在“佩德罗·库里”热带医学研究所诊断实验室收到的60份临床上疑似麻疹的配对血清进行了研究,这些血清来自麻疹、腮腺炎、风疹(MPR)疫苗的血清流行病学监测。使用克拉克实验室公司的麻疹IgM ELISA诊断试剂盒检测麻疹和风疹血凝抗体以及IgM。阳性病例通过IgM间接免疫荧光和中和试验进行确认。通过血凝抑制获得了3例麻疹和风疹阳性但麻疹IgM阴性的病例。由于这些病毒诱导多克隆反应的能力,还通过间接免疫荧光法(IIF)检测了针对爱泼斯坦-巴尔病毒、巨细胞病毒和单纯疱疹病毒的抗体。通过上述诊断试剂盒检测到6例IIF阴性的阳性病例。