[采用间接免疫荧光法、间接酶联免疫吸附测定法和反向酶联免疫吸附测定法对不同患者组中针对EB病毒（Epstein-Barr病毒）病毒衣壳抗原（VCA）的IgM抗体进行检测的比较。抗VCA EBV IgM酶联免疫吸附测定试剂盒（ELISA-VIDITEST）的诊断效能研究]

[A comparison of the detection of IgM antibodies against the viral capsid antigen (VCA) of EBV (Epstein-Barr virus) in various groups of patients, using indirect immunofluorescence, indirect ELISA and reverse ELISA. Study of the diagnostic efficacy of the anti-VCA EBV IgM ELISA-VIDITEST kit].

作者信息

Pumannová Markéta, Rezbová Martina, Svecová Miroslava, Hrbácková Hana, Novotná Markéta, Ochotná Jitka, Roubalová Katerina

机构信息

National ref. Laboratory for Herpesviruses, National Institute of Public Health, Prague, Czech Republic.

出版信息

Klin Mikrobiol Infekc Lek. 2004 Aug;10(4):186-90.

PMID:15328576

Abstract

OBJECTIVES

To test diagnostic efficiency of a novel method for detection of IgM antibodies to VCA EBV. To compare sensitivity and specificity of detection of IgM antibodies to VCA EBV from patients at various stages of EBV infection by various serological methods.

MATERIAL AND METHODS

IgM antibodies to VCA EBV were detected using IgM ELISA Viditest anti-VCA EBV IgM assay (Vidia, Ltd., Czech Republic) and comparative serological methods (indirect immunofluorescence assay, indirect ELISA (Human, SRN) and reverse ELISA (DiaSorin, Italy) in four independent diagnostic laboratories on panels of sera from 1) infectious mononucleosis patients, 2) patients with serological markers of EBV reactivation, 3) seropositive individuals lacking serological markers of active EBV infection, 4) seronegative individuals, 5) patients with IgM antibodies against another herpesvirus and 6) patients positive for rheumatoid factor. The sera yielding discrepant results were retested in the reference laboratory using the reference methods (indirect immunofluorescence and reverse ELISA). Overall, 854 IgM anti-VCA-positive or -negative sera were evaluated.

RESULTS

The sensitivity and specificity of the IgM Viditest anti-VCA EBV assay were 94.7 and 96.1 %, respectively. All of the three tests compared were similarly reliable in detecting IgM anti -VCA EBV antibodies in the samples from infectious mononucleosis patients. On the other hand, indirect fluorescence assay proved clearly superior to ELISAs for detection of IgM anti-VCA antibodies in the samples with serological pattern of EBV reactivation, typically showing significantly lower IgM anti-VCA titers compared with those from primary infection.

CONCLUSIONS

The multilaboratory comparative study proved that the novel IgM ELISA-Viditest anti-VCA EBV assay (Vidia, Ltd., Czech Republic) which shows comparable diagnostic efficiency for detection of IgM EBV antibodies to viral capsid antigen (VCA) as compared to the other assays tested, i.e. ELISA (Human, Germany) and reverse ELISA (DiaSorin, Italy), is suitable for use in serological diagnosis of EBV.

摘要

目的

检测一种检测抗VCA EBV IgM抗体的新方法的诊断效率。比较通过各种血清学方法检测处于EBV感染不同阶段患者的抗VCA EBV IgM抗体的敏感性和特异性。

材料与方法

在四个独立的诊断实验室中，使用IgM ELISA Viditest抗VCA EBV IgM检测法（Vidia有限公司，捷克共和国）以及比较血清学方法（间接免疫荧光法、间接ELISA（Human，SRN）和反向ELISA（DiaSorin，意大利））检测抗VCA EBV IgM抗体，检测对象为以下几组血清样本：1）传染性单核细胞增多症患者；2）具有EBV再激活血清学标志物的患者；3）缺乏活动性EBV感染血清学标志物的血清阳性个体；4）血清阴性个体；5）具有针对另一种疱疹病毒的IgM抗体的患者；6）类风湿因子阳性患者。对产生不一致结果的血清样本在参考实验室使用参考方法（间接免疫荧光法和反向ELISA）进行重新检测。总共评估了854份IgM抗VCA阳性或阴性血清样本。

结果

IgM Viditest抗VCA EBV检测法的敏感性和特异性分别为94.7%和96.1%。在检测传染性单核细胞增多症患者样本中的抗VCA EBV IgM抗体时，所比较的三种检测方法同样可靠。另一方面，在检测具有EBV再激活血清学模式的样本中的抗VCA IgM抗体时，间接荧光法明显优于ELISA法，这类样本中的抗VCA IgM滴度通常比原发性感染样本中的显著更低。

结论

多实验室比较研究证明，新型IgM ELISA - Viditest抗VCA EBV检测法（Vidia有限公司，捷克共和国）在检测抗病毒衣壳抗原（VCA）的IgM EBV抗体方面，与其他检测方法（即ELISA（德国Human公司）和反向ELISA（意大利DiaSorin公司））相比具有相当的诊断效率，适用于EBV的血清学诊断。