Arroyave C M
Centro de Diagnóstico Inmunológico, Monterrey, N. L., México.
Bol Med Hosp Infant Mex. 1993 Jun;50(6):399-405.
A procedure is described for the routine laboratory identification of IgM or/and IgG antibodies to measles and rubella simultaneously. A rapid dot-immunobinding assay on nitrocellulose was compared with ELISA systems. 58 serum samples from patients with an exanthematous disease and 15 serum negative samples for both virus were studied. The type of antibody found in all samples was variable and was always related to the presence of immunity in each individual. However, all known negative samples were negative. When results were compared with commercially available ELISA systems or reference laboratories, we found an excellent correlationship including titers. We concluded that the dot-immunobinding assay is a rapid, specific, sensitive, reproducible and economic test that can be used simultaneously for identification of several antigens.
本文描述了一种可同时常规实验室鉴定麻疹和风疹IgM或/及IgG抗体的方法。将硝酸纤维素膜上的快速斑点免疫结合试验与酶联免疫吸附测定（ELISA）系统进行了比较。研究了58例出疹性疾病患者的血清样本以及15例两种病毒检测均为阴性的血清样本。所有样本中发现的抗体类型各不相同，且始终与每个个体的免疫状态相关。然而，所有已知的阴性样本均为阴性。当将结果与市售ELISA系统或参考实验室的结果进行比较时，我们发现包括滴度在内的相关性极佳。我们得出结论，斑点免疫结合试验是一种快速、特异、灵敏、可重复且经济的检测方法，可同时用于多种抗原的鉴定。
