Nakajima K, Maeno Y, Honda A, Yokoyama K, Tooriyama T, Manabe S
National Research Institute of Aquaculture, Fisheries Agency, Mie, Japan.
Dis Aquat Organ. 1999 Apr 15;36(1):73-5. doi: 10.3354/dao036073.
Since 1990, red sea bream iridovirus (RSIV) has caused high mortalities in the summertime in cultured red sea bream Pagrus major in southwest Japan. To establish control measures for red sea bream iridoviral disease (RSIVD), the effectiveness of a formalin-killed viral vaccine was evaluated in a field trial. Two groups each consisting of 1000 juvenile red sea bream were either intraperitoneally inoculated with vaccine (vaccinated group) or were not vaccinated (non-vaccinated group). After vaccination, the fish were held for 1 wk, then transferred to a marine net pen and observed for 12 wk. The cumulative mortalities caused by RSIVD in the vaccinated group or control group were 19.2 and 68.5%, respectively. Additionally, the presence of virus antigen in the spleen was investigated and body weight was measured 6 and 12 wk post vaccination. In the vaccinated group, viral antigen was not detected. The increase in body weight of vaccinated fish was significantly (p < 0.05) greater than that of control fish. These results suggest that the vaccine against RSIVD was effective in 1 field trial.
自1990年以来，赤点石斑鱼虹彩病毒（RSIV）在日本西南部养殖的真鲷（Pagrus major）夏季造成了高死亡率。为了建立赤点石斑鱼虹彩病毒病（RSIVD）的防控措施，在一项田间试验中评估了福尔马林灭活病毒疫苗的有效性。两组各由1000尾真鲷幼鱼组成，一组腹腔接种疫苗（接种组），另一组不接种疫苗（未接种组）。接种疫苗后，将鱼饲养1周，然后转移到海水网箱中并观察12周。接种组或对照组由RSIVD引起的累积死亡率分别为19.2%和68.5%。此外，在接种疫苗后6周和12周时，检测脾脏中病毒抗原的存在情况并测量鱼的体重。在接种组中，未检测到病毒抗原。接种疫苗的鱼的体重增加显著（p<0.05）大于对照组鱼。这些结果表明，针对RSIVD的疫苗在一项田间试验中是有效的。
