Caldwell M C, Datta S
Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843-2128, USA.
Mech Dev. 1998 Dec;79(1-2):121-30. doi: 10.1016/s0925-4773(98)00178-6.
The trol locus of Drosophila regulates the timing of neuroblast proliferation. In trol mutants, quiescent neuroblasts fail to begin division. We have investigated this cell cycle arrest to examine trol function. Induced expression of cyclin E or DP/E2F in trol mutants results in normal levels of dividing neuroblasts, while cyclin B expression has no effect. cyclin E expression is lower in the trol mutant larval CNS as assayed by quantitative RT-PCR, suggesting that trol neuroblasts are arrested in G1 due to lack of Cyclin E. Neither cyclin E nor E2F expression can phenocopy ana mutations, indicating that arrest caused by lack of Trol is different from Ana-mediated arrest.
果蝇的trol基因座调节神经母细胞增殖的时间。在trol突变体中，静止的神经母细胞无法开始分裂。我们研究了这种细胞周期停滞以检验trol的功能。在trol突变体中诱导表达细胞周期蛋白E或DP/E2F会导致分裂神经母细胞达到正常水平，而细胞周期蛋白B的表达则没有影响。通过定量逆转录-聚合酶链反应检测发现，trol突变体幼虫中枢神经系统中细胞周期蛋白E的表达较低，这表明trol神经母细胞因缺乏细胞周期蛋白E而停滞在G1期。细胞周期蛋白E和E2F的表达均不能模拟ana突变，这表明因缺乏Trol导致的停滞与Ana介导的停滞不同。
