Wiederholt K, McLaughlin L W
Merkert Chemistry Center, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02167, USA.
Nucleic Acids Res. 1999 Jun 15;27(12):2487-93. doi: 10.1093/nar/27.12.2487.
A non-nucleoside linker based upon the ligand 2,2'-bipyridine and ethylene glycol is prepared and placed into the backbone of a number of oligonucleo-tides. The bipyridine ligand is reacted with cis -dichloro bis(2,2'-bipyridyl) Ru(II) to generate the relatively substitutionally inert complex based upon the well-characterized tris -2,2'-bipyridyl Ru(II). The ruthenium-containing DNA complexes exhibited UV and fluorescence characteristics that are consistent with those previously observed for simple tris -2,2'-bipyridyl Ru(II) complexes. Oligonucleotides containing the ruthenium complex will form both DNA duplexes and triplexes with stabilities that are slightly better than those formed from simple tethered oligonucleotide probes in which the two hybridizing sequences are tethered by simple tri(ethylene glycol) or hexa(ethylene glycol) linkers.
制备了一种基于配体2,2'-联吡啶和乙二醇的非核苷连接体，并将其置于多种寡核苷酸的主链中。联吡啶配体与顺式二氯双(2,2'-联吡啶)钌(II)反应，生成基于特征明确的三(2,2'-联吡啶)钌(II)的相对取代惰性配合物。含钌的DNA配合物表现出的紫外和荧光特性与先前观察到的简单三(2,2'-联吡啶)钌(II)配合物的特性一致。含有钌配合物的寡核苷酸将形成DNA双链体和三链体，其稳定性略优于由简单的三(乙二醇)或六(乙二醇)连接体连接两个杂交序列的简单连接寡核苷酸探针形成的双链体和三链体。
