Greene W K, Baker E, Rabbitts T H, Kees U R
TVW Telethon Institute for Child Health Research, Division of Childrens' Leukaemia and Cancer Research, West Perth, WA 6872, Australia.
Gene. 1999 May 31;232(2):203-7. doi: 10.1016/s0378-1119(99)00125-0.
Human SLIM1 is a recently described gene of the LIM-only class encoding four and a half tandemly repeated LIM domains. LIM domains are double zinc finger structures which provide an interface for protein/protein interactions and are conserved in a variety of nuclear and cytoplasmic factors important in cell fate determination and cellular regulation. Here we report the structural organization, expression pattern and chromosomal localization of the human SLIM1 gene. SLIM1 was found to contain at least five exons with all four introns disrupting the coding region at a similar position relative to the respective complete LIM domains. Northern blot analysis confirmed strikingly high expression of SLIM1 in skeletal muscle and heart, with much lower expression observed in several other tissues including colon, small intestine and prostate. The SLIM1 gene was assigned to human chromosome Xq26 using fluorescence in situ hybridization.
人类SLIM1是一种最近描述的仅含LIM结构域的基因，编码四个半串联重复的LIM结构域。LIM结构域是双锌指结构，为蛋白质/蛋白质相互作用提供界面，并且在多种对细胞命运决定和细胞调节至关重要的核因子和细胞质因子中保守。在此，我们报告人类SLIM1基因的结构组织、表达模式和染色体定位。发现SLIM1至少包含五个外显子，所有四个内含子在相对于各自完整LIM结构域的相似位置破坏编码区。Northern印迹分析证实SLIM1在骨骼肌和心脏中表达显著高，而在包括结肠、小肠和前列腺在内的其他几种组织中表达低得多。使用荧光原位杂交将SLIM1基因定位于人类X染色体q26。
