Rafael E, Wernerson A, Arner P, Tibell A
Department of Transplantation Surgery, Karolinska Institute, Huddinge Hospital, Stockholm, Sweden.
Eur Surg Res. 1999;31(3):249-58. doi: 10.1159/000008700.
In this study, insulin was injected into Theracyte immunoisolation devices to analyze changes in the permeability of the device over time after implantation. The recovery of insulin was studied after subcutaneous implantation of the devices in rats, using the microdialysis technique. The area under the insulin concentration vs. time curves (AUC) after insulin injection in devices implanted 1 day previously did not differ significantly from the AUC after subcutaneous injection. At 1, 2 and 4 weeks after implantation, the recovery of insulin was significantly reduced, but at 3 months, the AUC was not significantly different from that in the control group. Histological examination showed that the number of vascular profiles within 15 microm of the device were significantly higher at 2, 4 weeks and 3 months after transplantation when compared to numbers at 1 week. The design of the device allows transplantation of cells at a chosen time point after its implantation. Delayed filling of the device would allow neovascularization of the device surface before graft implantation and we suggest that such a schedule might improve function of the encapsulated graft.
在本研究中，将胰岛素注入Theracyte免疫隔离装置，以分析植入后该装置的通透性随时间的变化。使用微透析技术，在大鼠皮下植入该装置后研究胰岛素的回收率。在先前植入1天的装置中注射胰岛素后，胰岛素浓度与时间曲线下面积（AUC）与皮下注射后的AUC无显著差异。植入后1、2和4周，胰岛素的回收率显著降低，但在3个月时，AUC与对照组无显著差异。组织学检查显示，与移植后1周相比，移植后2、4周和3个月时，装置15微米范围内的血管轮廓数量显著增加。该装置的设计允许在植入后选定的时间点移植细胞。装置的延迟填充将允许在移植物植入前使装置表面形成新血管，我们认为这样的安排可能会改善封装移植物的功能。
