Lacki J K, Korczowska I, Mackiewicz S H
Department of Rheumatology, Karol Marcinkowski University School of Medical Sciences, Poznan, Poland.
J Investig Allergol Clin Immunol. 1999 Mar-Apr;9(2):96-100.
In order to better understand the immunological mechanisms involved in the pathogenesis of rheumatoid arthritis (RA), the level of various lymphocyte subsets in the peripheral blood of 29 patients with erosive RA was determined. All the patients were treated with methotrexate for 2 years. The total number and the proportion of CD3 cells, CD3+CD4+ and CD3+CD8+ cells did not change during the study. The initially increased level of CD19+ B-cells and CD19+CD5+ cells decreased during the treatment. The percentage of CD3-CD16+ natural killer cells was not affected by the treatment. At the inception of the study, we observed a deficiency of CD4+ CD45RA+ cells and the level of CD3+CD29+ cells was slightly increased. During the treatment we noticed significant elevation of CD45RA cells. Consequently, the CD29/CD45RA ratio significantly decreased. We showed significant correlation between changes in disease activity and changes in the level of CD19+ cells and CD4+CD29+ cells. Our results suggest that low-dose methotrexate may affect immunocompetent cells. The lowering in the CD29+ subset population associated with depletion of CD19 B-cells after methotrexate therapy may limit abnormal CD4+ cell activation and reduce the migration of lymphocytes into inflamed synovium.
为了更好地理解类风湿关节炎（RA）发病机制中涉及的免疫机制，我们测定了29例侵蚀性RA患者外周血中各种淋巴细胞亚群的水平。所有患者均接受甲氨蝶呤治疗2年。研究期间，CD3细胞、CD3 + CD4 +和CD3 + CD8 +细胞的总数及比例未发生变化。治疗期间，最初升高的CD19 + B细胞和CD19 + CD5 +细胞水平下降。CD3 - CD16 +自然杀伤细胞的百分比不受治疗影响。在研究开始时，我们观察到CD4 + CD45RA +细胞缺乏，且CD3 + CD29 +细胞水平略有升高。治疗期间，我们注意到CD45RA细胞显著升高。因此，CD29/CD45RA比值显著降低。我们发现疾病活动度的变化与CD19 +细胞和CD4 + CD29 +细胞水平的变化之间存在显著相关性。我们的结果表明，低剂量甲氨蝶呤可能影响免疫活性细胞。甲氨蝶呤治疗后，与CD19 B细胞耗竭相关的CD29 +亚群数量减少可能会限制异常CD4 +细胞的激活，并减少淋巴细胞向炎症滑膜的迁移。
