Khan Z U, Chugh T D, Chandy R, Provost F, Boiron P
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Mycopathologia. 1998;143(3):151-4. doi: 10.1023/a:1006901230367.
In this study, using the API-ZYM system, we have reported the enzyme profile of 42 soil strains and 2 clinical strains of Nocardia asteroides isolated locally. Of the 19 enzymes tested, only 7 were demonstrable in over 90% of the soil isolates. These included alkaline phosphatase, esterase lipase, leucine arylamidase, acid phosphatase, phosphohydrolase, alpha-glucosidase and beta-glucosidase. In addition, beta-galactosidase activity was demonstrated in all the strains by the O-nitrophenyl-beta-D-galactopyranoside (ONPG) test. The enzymes which were not demonstrable in > 95% of the strains included valine arylamidase, cystine arylamidase, trypsin, chymotrypsin, alpha-galactosidase, beta-glucoronidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase and alpha-fucosidase. With the exception of valine arylamidase, which was lacking in all but one isolate, the enzyme profiles of the soil isolates were comparable with the clinical isolates of N. asteroides reported in previous studies. The reasons for this difference in the two sets of isolates is not clear. The study reinforces the view that specific differences in the enzymatic profiles of Nocardia species could be used for their rapid identification. However, more extensive studies are needed to establish the reproducibility of this method. To the best of our knowledge, this is the first study of the enzymatic profile of soil isolates of N. asteroides originating from a single geographic region.
在本研究中，我们使用API-ZYM系统报告了本地分离出的42株土壤菌株和2株临床星形诺卡菌菌株的酶谱。在所测试的19种酶中，只有7种在超过90%的土壤分离株中可检测到。这些酶包括碱性磷酸酶、酯酶脂肪酶、亮氨酸芳基酰胺酶、酸性磷酸酶、磷酸水解酶、α-葡萄糖苷酶和β-葡萄糖苷酶。此外，通过邻硝基苯-β-D-吡喃半乳糖苷（ONPG）试验在所有菌株中均检测到β-半乳糖苷酶活性。在超过95%的菌株中未检测到的酶包括缬氨酸芳基酰胺酶、胱氨酸芳基酰胺酶、胰蛋白酶、糜蛋白酶、α-半乳糖苷酶、β-葡萄糖醛酸酶、N-乙酰-β-葡萄糖胺酶、α-甘露糖苷酶和α-岩藻糖苷酶。除了缬氨酸芳基酰胺酶在除一株分离株外的所有菌株中均缺乏外，土壤分离株的酶谱与先前研究中报道的临床星形诺卡菌分离株相当。两组分离株存在这种差异的原因尚不清楚。该研究强化了这样一种观点，即诺卡菌属酶谱的特定差异可用于其快速鉴定。然而，需要更广泛的研究来确定该方法的可重复性。据我们所知，这是首次对来自单一地理区域的土壤星形诺卡菌分离株的酶谱进行研究。
