Williams C M, Kirkham T C
Department of Psychology, University of Reading, Whiteknights, UK.
Psychopharmacology (Berl). 1999 Apr;143(3):315-7. doi: 10.1007/s002130050953.
Central cannabinoid systems have been implicated in appetite regulation by the respective hyperphagic actions of exogenous cannabinoids, such as delta9-THC, and hypophagic effects of selective cannabinoid receptor antagonists.
This study examined whether an endogenous cannabinoid, anandamide, could induce overeating, via a specific action at central (CB1) cannabinoid receptors.
Pre-satiated male rats (n=18), received subcutaneous injections of anandamide (0.5, 1.0, 5.0, 10.0 mg/kg) before 3-h, nocturnal food intake tests. In a second series of intake tests (n=8), anandamide injection (1.0 mg/kg) was preceded by injection of the specific CB1 receptor antagonist, SR141716 (0.1, 0.5, 1.0 mg/kg SC).
All doses of anandamide induced significant overeating, with 1.0 mg/kg being most potent. Additionally, hyperphagia induced by 1.0 mg/kg anandamide was dose-dependently attenuated by SR141716 pretreatment.
This first demonstration of anandamide-induced, CB -mediated, overeating provides important evidence for the involvement of a central cannabinoid system in the normal control of eating.
中枢大麻素系统与食欲调节有关，外源性大麻素（如δ9-四氢大麻酚）具有促食欲作用，而选择性大麻素受体拮抗剂则有抑制食欲的作用。
本研究旨在探讨内源性大麻素花生四烯乙醇胺是否通过作用于中枢（CB1）大麻素受体而诱导暴饮暴食。
在夜间3小时进食测试前，给预饱的雄性大鼠（n = 18）皮下注射花生四烯乙醇胺（0.5、1.0、5.0、10.0 mg/kg）。在另一系列进食测试（n = 8）中，在注射花生四烯乙醇胺（1.0 mg/kg）之前，先注射特异性CB1受体拮抗剂SR141716（0.1、0.5、1.0 mg/kg，皮下注射）。
所有剂量的花生四烯乙醇胺均诱导显著的暴饮暴食，其中1.0 mg/kg最为有效。此外，SR141716预处理可剂量依赖性地减弱1.0 mg/kg花生四烯乙醇胺诱导的食欲亢进。
花生四烯乙醇胺诱导的、CB介导的暴饮暴食的首次证明为中枢大麻素系统参与正常饮食控制提供了重要证据。
