Chambers G K, McDonald J F, McElfresh M, Ayala F J
Biochem Genet. 1978 Aug;16(7-8):757-67. doi: 10.1007/BF00484733.
Starch and polyacrylamide gel electrophoresis were used to ascertain the substrate specificities of alcohol-oxidizing enzymes in 13 Drosophila species. The substrates used were a variety of long- and short-chain aliphatic alcohols, one aromatic alcohol, and benzaldehyde. Only one enzyme (product of a single-gene locus) showed significant NAD+-dependent alcohol dehydrogenase activity with short-chain aliphatic alcohols. The 13 species, belonging to four different Drosophila groups, all showed a similar complement of alcohol-oxidizing enzymes, although differences in electrophoretic mobility and in levels of activity existed from species to species. These findings are relevant to the adaptation of Drosophila to alcohol environments.
采用淀粉凝胶电泳和聚丙烯酰胺凝胶电泳来确定13种果蝇中酒精氧化酶的底物特异性。所使用的底物包括多种长链和短链脂肪醇、一种芳香醇以及苯甲醛。只有一种酶（单个基因座的产物）对短链脂肪醇表现出显著的依赖NAD⁺的酒精脱氢酶活性。这13个物种分属于四个不同的果蝇类群，尽管不同物种之间在电泳迁移率和活性水平上存在差异，但它们都表现出相似的酒精氧化酶组合。这些发现与果蝇对酒精环境的适应性相关。
