Hergt R, El-Washali A, Sarie N
Z Erkr Atmungsorgane. 1976;145(3):294-8.
Sera of patients, blood donors and cattle sera were examined by means of diffusion--in--gel technique using self produced antigens, prepared from H 37 Ra, one fresh isolated strain of M. tbc and BCG Jena. 38.2% of 421 patients with pulmonary tuberculosis reacted positive. Positive reactions were observed in 55.7% of 199 patients suffering from another infectious disease and in 31;7% of normal sera. Most precipitation lines were found with the antigen prepared from H37 Ra after incubation for 7 months. In human sera as well as in cattle a dependence of reactions upon mode of antigen production was evident.
使用从H 37 Ra(一株新分离的结核分枝杆菌菌株)、卡介苗耶拿株制备的自制抗原,通过凝胶扩散技术检测患者血清、献血者血清和牛血清。421例肺结核患者中有38.2%反应呈阳性。199例患有其他传染病的患者中有55.7%观察到阳性反应,正常血清中有31.7%呈阳性反应。孵育7个月后,用从H37 Ra制备的抗原发现的沉淀线最多。在人血清和牛血清中,反应对抗原生产方式的依赖性都很明显。