Britton W J, Hellqvist L, Garsia R J, Basten A
Clinical Immunology Research Centre, University of Sydney, NSW, Australia.
Clin Exp Immunol. 1988 Mar;71(3):394-8.
Mycobacterial antigens which react with human B lymphocytes were investigated by immunoprecipitation of radiolabelled sonicates of Mycobacterium leprae and M. bovis (BCG) with sera from patients with leprosy and tuberculosis in the presence of Staphylococcus aureus. SDS-PAGE analysis of the immunoprecipitates demonstrated that dense bands of Mr 12,000 (12K), 15K, 27K, 32-33K, 36K and 48K were the major antigens of M. leprae recognized by antibodies in lepromatous leprosy sera. Of these, only the 15-16K band reacted significantly with sera from patients with tuberculoid leprosy and tuberculosis. Other antigens including the T cell immunogens of Mr 18K and 70K reacted with some of the BL/LL sera tested. There were differences in the pattern of antigens precipitated from BCG sonicate by leprosy sera with the 65K antigen and a high molecular weight band (greater than 94K) being readily detected. These results differ in part to these obtained by probing immunoblots of M. leprae sonicate with leprosy sera. Factors contributing to these differences are discussed.
在金黄色葡萄球菌存在的情况下，用麻风病患者和结核病患者的血清对麻风分枝杆菌和牛分枝杆菌（卡介苗）的放射性标记超声裂解物进行免疫沉淀，以研究与人类B淋巴细胞发生反应的分枝杆菌抗原。对免疫沉淀物进行的SDS - PAGE分析表明，分子量为12,000（12K）、15K、27K、32 - 33K、36K和48K的致密条带是瘤型麻风血清中抗体识别的麻风分枝杆菌的主要抗原。其中，只有15 - 16K条带与结核样型麻风患者和结核病患者的血清有明显反应。包括分子量为18K和70K的T细胞免疫原在内的其他抗原与部分检测的BL/LL血清发生反应。麻风血清从卡介苗超声裂解物中沉淀出的抗原模式存在差异，65K抗原和一条高分子量条带（大于94K）很容易被检测到。这些结果与用麻风血清探测麻风分枝杆菌超声裂解物的免疫印迹所获得的结果部分不同。文中讨论了导致这些差异的因素。
