Yelon D, Stainier D Y
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448, USA.
Semin Cell Dev Biol. 1999 Feb;10(1):93-8. doi: 10.1006/scdb.1998.0278.
A classical genetic approach, in which mutagenized organisms are screened for phenotypes of interest, is appealing for the analysis of developmental processes. Here, we describe the advantages of zebrafish genetics for the study of heart development. As an example of the utility of this strategy, we discuss its potential to illuminate the molecular mechanisms of cardiac chamber formation. The signals that specify ventricular and atrial lineages and the differentiation pathways that produce distinct chambers are poorly understood. Recently identified zebrafish mutations that disrupt ventricular or atrial development promise to reveal genes essential for these processes.
一种经典的遗传学方法，即对诱变生物体进行感兴趣的表型筛选，对于发育过程的分析很有吸引力。在这里，我们描述了斑马鱼遗传学在心脏发育研究中的优势。作为该策略实用性的一个例子，我们讨论其阐明心腔形成分子机制的潜力。确定心室和心房谱系的信号以及产生不同心腔的分化途径目前还了解甚少。最近发现的破坏心室或心房发育的斑马鱼突变有望揭示这些过程所必需的基因。
