Akiba M, Sameshima T, Nakazawa M
Laboratory of Zoonosis, Feed Safety Research Division, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
Epidemiol Infect. 1999 Apr;122(2):343-6. doi: 10.1017/s0950268899002204.
A total of 46 Escherichia coli O157:H7 isolates were obtained from sequential faecal samples from seven cattle collected over periods of 2 months. Nine closely related genetic subtypes, determined by pulsed-field gel electrophoresis types using three kinds of restriction endonuclease were observed among the isolates. Distinguishable, but closely related genetic subtypes can be isolated from one farm, or from one cow, should be considered when undertaking an epidemiological survey.
从7头牛连续2个月采集的粪便样本中总共获得了46株大肠杆菌O157:H7分离株。使用三种限制性内切酶通过脉冲场凝胶电泳分型确定了9种密切相关的基因亚型。在进行流行病学调查时，应考虑可从一个农场或一头牛中分离出可区分但密切相关的基因亚型。
