Akiba M, Masuda T, Sameshima T, Katsuda K, Nakazawa M
Laboratory of Zoonosis, Feed Safety Research Division, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
Epidemiol Infect. 1999 Apr;122(2):337-41. doi: 10.1017/s0950268899002198.
A total of 77 Escherichia coli O157:H7 (H-) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx-2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157:H7 infection in Japan.
采用分子生物学方法对日本77株来自牛的大肠杆菌O157:H7（H-）分离株进行了研究。这些分离株大多（43株）携带stx-2基因，但不携带stx1基因。观察到15种噬菌体类型和50种脉冲场凝胶电泳（PFGE）图谱。通过这些方法，有1株分离株与人类暴发菌株无法区分。这表明在日本，牛必须被视为人类感染大肠杆菌O157:H7的可能来源。
